Figure 2.

MRPL23-AS1 promotes OS cell proliferation and invasion in vitro and in vivo. (a,b) qRT-PCR verifying the construction of stable MRPL23-AS1-overexpressing 143B cells and MRPL23-AS1-depletd U2OS cells. (c) CCK-8 assay detecting the viability of OS cells after alteration of MRPL23-AS1 level. (d,e) Transwell assay analyzing cell migration and invasion after MRPL23-AS1 knockdown or overexpression. (f) The representative image of nude mice bearing control or MRPL23-AS1-silenced U2OS cells. (g) Bioluminescence imaging showing the effect of MRPL23-AS1 knockdown on in vivo lung metastasis of U2OS cells. *p < 0.05, **p < 0.01, ***p < 0.001