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. 2021 Jan 11;12(1):369–381. doi: 10.1080/21655979.2020.1868742

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — IRAK1 is the downstream target of miR-146a-5p in PDAC

A. IRAK1-WT, IRAK1-MUT1, IRAK1-MUT2, and IRAK1-MUT1&2 luciferase reporter vectors were constructed.B. MiR-146a-5p mimics were co-transfected with IRAK1-WT, IRAK1-MUT1, IRAK1-MUT2, or IRAK1-MUT1&2, respectively, into 293 T cells, and then the luciferase activity of the cells in each group was determined.C. MiR-146a-5p mimic, miR-146a-5p inhibitor, and control miRNAs were respectively transfected into BxPC-3 and Colo357 cells, and the expression of miR-146a-5p in the two cell lines was detected by qRT-PCR.D. IRAK1 expression at mRNA expression level in BxPC-3 and Colo357 cells was detected by qRT-PCR after the transfection.E. Western blot assay was used to detect the expression of IRAK1 in BxPC-3 and Colo357 cells after the transfection.* P < 0.05, ** P < 0.01, and *** P < 0.001.