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. 2021 May 23;12(1):1952–1963. doi: 10.1080/21655979.2021.1921553

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of circ_0062582 on the osteogenic differentiation of hBMSCs by regulating miR-145/CBFB pathway. (a) Expression levels of CBFB in hBMSCs. (b) Expression levels of CBFB in hBMSCs following transfection with the CBFB overexpression vector. *P < 0.05, **P < 0.01. (c) Viability of hBMSCs. ***P < 0.001 vs. OM+pcDNA-NC; ^##P < 0.01 and ^###p < 0.001 vs. OM+pcDNA-circ_0062582+ miR-NC

^@@P < 0.01 and ^@@@P < 0.001 vs. OM+pcDNA-circ_0062582+ miR-145 mimic+OE-NC. (D) Alkaline phosphatase staining was used to detect the differentiation activity of hBMSCs. Magnification, x200. (E) Alizarin red staining was used to detect the mineralization levels of hBMSCs. Magnification, x200. (F) Protein expression levels of OSX, OCN and COL1. ***P < 0.001. CBFB, core-binding factor subunit β; hBMSCs, human bone marrow mesenchymal stem cells; OSX, osterix; OCN, osteocalcin; COL1, collagen type 1.