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Schematic detailing the experimental culture routes investigated. Cells in each route were seeded according to the density stated in the figure. Following 48 h culture route A1, A3, and B1 were subjected to a 100% medium exchange. Following 72 h, all routes were passaged. Route A1, A2, A3 and A4 underwent a further two passages (experiment A). Route B1 and B2 underwent a further nine passages (experiment B); *M. Ex = medium exchange
