Table 1.

Summary of the significance values at each passage, comparing the SMRs of the different culture routes using Tukey’s multiple comparisons test. Both seeding density and feeding regime are shown to result in significant difference between the four routes. The highest significant differences between routes, within passages, are observed when both parameters are changed, e.g. A2 vs A3 (n = 2 for each condition)

		Passage 1		Passage 2		Passage 3
Condition	Parameter	Summary	P Value	Summary	P Value	Summary	P Value
A1 vs. A2	A1 vs. A2	*	0.024	**	0.0082	**	0.0059
A1 vs. A3	A1 vs. A3	*	0.0494	**	0.0026	ns	0.0573
A1 vs. A4	A1 vs. A4	ns	0.998	*	0.0438	ns	0.1793
A2 vs. A3	A2 vs. A3	***	0.0002	****	<0.0001	****	<0.0001
A2 vs. A4	A2 vs. A4	*	0.0327	****	<0.0001	ns	0.2435
A3 vs. A4	A3 vs. A4	*	0.0364	ns	0.3936	**	0.0013