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. 2021 May 6;12(1):1599–1610. doi: 10.1080/21655979.2021.1920323

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — LncRNA PP7080 regulates HCC by targeting miR-601

(a) The targeted binding site of lncRNA PP7080 is predicted online by DIANA TOOLS software, the binding site is displayed and the mutation sequence of the site is designed. (b) The above-mentioned binding site and the mutated sequence were constructed into the fluorescein reporter gene, and the expression of fluorescein was detected after HepG2 or Huh7 was transfected with miR-NC or miR-601, respectively. (c) The effect of knockdown of lncRNA PP7080 on the expression of miR-601 was detected by qRT-PCR. (d) The expression levels of miR-601 in 40 pairs of HCC cancer tissues and adjacent tissues were detected by qRT-PCR. (e) The Pearson correlation analysis of the expression of lncRNA PP7080 and miR-601 in the cancer tissues of 40 cancer patients. Data are representative of three independent experiments and expressed as mean ± SD (***p < 0.001).