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. 2021 May 6;12(1):1599–1610. doi: 10.1080/21655979.2021.1920323

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — LncRNA PP7080 promotes the malignant progression of HCC through the miR-601/SIRT1 signal axis

(a) The inhibition efficiency of miR-601inh on mir-601 was detected by qRT-PCR. (b) Transfection efficiency test of SIRT1 overexpression in HepG2 or Huh7, the expression level of SIRT1 mRNA was detected by qRT-PCR. (c, d) Detection of the expression of SIRT1 protein in HepG2 or Huh7 after cells were co-transfected with miR-601inh or OE-SIRT1 after lncRNA PP7080 knockdown by WB of sh-NC group, sh-PP7080#1 group, sh-PP7080#1+ miR-601inh group and sh-PP7080#1+ OE-SIRT1 group. (e, f) Co-transfected with miR-601inh or OE-SIRT1 after lncRNA PP7080 knockdown induced HepG2 or Huh7 cell viability detection by CCK8 assay of sh-NC group, sh-PP7080#1 group, sh-PP7080#1+ miR-601inh group and sh-PP7080#1+ OE-SIRT1 group. (g) The effect of co-transfected with miR-601inh or OE-SIRT1 after lncRNA PP7080 knockdown on Huh7 and HepG2 cell colony forming detected by transwell experiment. (h) The effect of co-transfected with miR-601inh or OE-SIRT1 after lncRNA PP7080 knockdown on Huh7 and HepG2 cell migration detected by transwell experiment. (i) The effect of co-transfected with miR-601inh or OE-SIRT1 after lncRNA PP7080 knockdown on Huh7 and HepG2 cell invasion detected by transwell experiment (with Matrigel). Data are representative of three independent experiments and expressed as mean ± SD (**p < 0.01; ***p< 0.001).