Figure 3.

METTL3 is the target of miR-1269b A) The predicted binding sequence between METTL3 mRNA 3ʹUTR and miR-1269b. (b) Dual-luciferase reporter gene assay was used to verify the binding relationship between METLL3 mRNA 3ʹ UTR and miR-1269b. (c) and (d) qRT-PCR and Western blot were used to detect the expression of METTL3 mRNA and protein in NCI-N87 and SNU-16 cells transfected with miR-1269b mimics or inhibitors. (e) qRT-PCR was used to detect the expression of METTL3 mRNA in GC tissues and adjacent tissues. (f) Pearson correlation was used to analyze the correlation between METTL3 mRNA expression and miR-1269b expression in GC tissues. **P < 0.01, and ***P < 0.001