Figure 1.

The efficiency of overexpressed ITGβ3 vector and ITGβ3 siRNAs was evaluated in mouse podocyte cell line MPC5. (a) ITGβ3 expression in NC and ITGβ3 group was tested by RT-qPCR, (b) ITGβ3 expression in si-NC, si-ITGβ3-1 and si-ITGβ3-2 group was detected by RT-qPCR. (c) ITGβ3 protein expression was detected by Western blot analysis. Data are shown as mean ± standard deviation, N = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.001

Abbreviations: NC: negative control of ITGβ3 overexpression vector; ITGβ3: the group of transfection with integrin β3 overexpression vector; si-NC: small interfering negative control; si-ITGβ3-1: small interfering integrin β3 RNA-1; si-ITGβ3-2: small interfering integrin β3 RNA-2.