Figure 4.

ITGβ3 overexpression inhibits the RhoA-YAP pathway in order to promote podocyte injury progression. (a). Wound healing analysis in vitro assay was used to determine the ability of wound closure in MPC5 cells after transfection with NC, ITGβ3, or ITGβ3+ RhoA. (b). Flow cytometry was used to test apoptosis in MPC5 cells after transfection with NC, ITGβ3 or ITGβ3+ RhoA. (c). RT-qPCR was used to evaluate ITGβ3, RhoA, and YAP mRNA expressions in MPC5 cells after transfection with NC, ITGβ3 or ITGβ3+ RhoA. (d). Western blotting was used to determine ITGβ3, RhoA, total YAP, and nuclear YAP in MPC5 cells after transfection with NC, ITGβ3 or ITGβ3+ RhoA. Data are shown as mean ± standard deviation, N = 3. *, p < 0.05; NC: negative control of ITGβ3 overexpression vector; ITGβ3: integrin β3; ITGβ3+ RhoA: the group of cotransfection with integrin β3 overexpression vector and Ras homolog gene family, member A overexpression vector

Abbreviations: RT-qPCR: reverse transcription-quantitative polymerase chain reaction; RhoA: Ras homolog gene family, member A; YAP: Yes-associated protein.