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SMAD5 silencing promoted AGS cells apoptosis. (a) The cell apoptosis rate was determined by flow cytometry. (b) The expression levels of Bcl-2, Bax, Caspase-3 and pro-Caspase-3 were determined by western blot, the GAPDH was set as the internal normalization control. Error bars represent the mean ± SEM from three independent experiments. ***P< 0.001 vs. Control
