Figure 2.

Effect of STAT3 on the transcriptional activity of FTO promoter and the expression of FTO in doxorubicin-resistant BC cells and their parental cells. a The binding sites of STAT3 in FTO promoter predicted by JASPAR. b CHIP assay confirmed the binding between FTO promoter and STAT3 in doxorubicin-resistant MCF-7 cells. c, d Dual luciferase reporter assay confirmed that the transcriptional activity of FTO promoter was inhibited by S3I-201 but enhanced by EGF in doxorubicin-resistant MCF-7 cells and MCF-7 cells. e-l The mRNA and protein expression of FTO was evaluated using RT-qPCR and western blotting. *p < 0.05, **p < 0.01. MCF-7-DoxR, doxorubicin-resistant MCF-7; MDA-MB-231-DoxR, doxorubicin-resistant MDA-MB-231; EGF, epidermal growth factor