Figure 4.

Role of STAT3-FTO in the doxorubicin-induced loss of BC cell viability. Doxorubicin-resistant BC or BC cells were transfected with FTO siRNAs or FTO overexpression plasmids and co-transfected with FTO siRNA and FTO overexpression plasmids for 48 h and then exposed to doxorubicin for 24 h. a-f Cell viability was measured by CCK-8 assay. *p < 0.05, **p < 0.01. MCF-7-DoxR, doxorubicin-resistant MCF-7; MDA-MB-231-DoxR, doxorubicin-resistant MDA-MB-231; siNC, negative control siRNA; siFTO, FTO siRNA; NC-OE, empty vector; OE-FTO, FTO overexpression; OD, optical density