Figure 8.

Role of STAT3-FTO in the doxorubicin-induced viability loss and apoptosis of Hs578T cells. Hs578T cells were transfected with FTO overexpression plasmids and co-transfected with STAT3 overexpression plasmids and FTO siRNA-1 for 48 h and then exposed to doxorubicin for 24 h. a, b Cell viability was measured by CCK-8 assay. c, d Apoptotic Hs578T cells were detected by Annexin V/PI staining. *p < 0.05, **p < 0.01. Dox, doxorubicin; OD, optical density; PI, propidium iodide; NC-OE, empty vector; OE-FTO, FTO overexpression; OE-STAT3, STAT3 overexpression; siNC, negative control siRNA; siFTO-1, FTO siRNA-1