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. 2020 Dec 31;12(1):196–207. doi: 10.1080/21655979.2020.1860492

Table 2.

qPCR primer sequences

Gene Primer sequences (5'-3')
PGM5P4-AS1 (F) GGGGCAGAGGGACTATGTT
PGM5P4-AS1 (R) GAGGCTGAGGTGGGAGGAT
LZTS3 (F) CACCGCAGTATCGTGAGCC
LZTS3 (R) GGAAATTCTTGGGTACGACAGG
GAPDH (F) GACCTGACCTGCCGTCTAG
GAPDH (R) AGGAGTGGGTGTCGCTGT
hsa-miR-1275 (F) GAACCTGGTAGTGGGGGAGA
hsa-miR-1275 (R) GTGCAGGGTCCGAGGTATTC
U6 (F) GCTTCGGCAGCACATATACT
U6 (R) GTGCAGGGTCCGAGGTATTC

Note: F, forward; R, reverse.

PGM5P4-AS1 inhibited the growth of tumors in nude mice and suppressed the abilities of cell proliferation, migration as well as invasion of lung cancer cells. In mechanism, PGM5P4-AS1 performed antitumor function by up-regulating the expression of LZTS3 via sponging miR-1275.