Table 2.
Gene | Primer sequences (5'-3') |
---|---|
PGM5P4-AS1 (F) | GGGGCAGAGGGACTATGTT |
PGM5P4-AS1 (R) | GAGGCTGAGGTGGGAGGAT |
LZTS3 (F) | CACCGCAGTATCGTGAGCC |
LZTS3 (R) | GGAAATTCTTGGGTACGACAGG |
GAPDH (F) | GACCTGACCTGCCGTCTAG |
GAPDH (R) | AGGAGTGGGTGTCGCTGT |
hsa-miR-1275 (F) | GAACCTGGTAGTGGGGGAGA |
hsa-miR-1275 (R) | GTGCAGGGTCCGAGGTATTC |
U6 (F) | GCTTCGGCAGCACATATACT |
U6 (R) | GTGCAGGGTCCGAGGTATTC |
Note: F, forward; R, reverse.
PGM5P4-AS1 inhibited the growth of tumors in nude mice and suppressed the abilities of cell proliferation, migration as well as invasion of lung cancer cells. In mechanism, PGM5P4-AS1 performed antitumor function by up-regulating the expression of LZTS3 via sponging miR-1275.