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. 2021 Feb 8;12(1):565–577. doi: 10.1080/21655979.2021.1880732

Figure 5.

Figure 5.

miR-6727-5p targeted and decreased TBX1 expression in SiHa cells. (a) Potential binding site between miR-6727-5p and TBX1 was predicted using TargetScan database and validated by dual luciferase reporter assay. (b, c) Relative expression of miR-6727-5p and TBX1 mRNA were detected by qRT-PCR. (d) Expression level of TBX1 protein was detected in SiHa cells by Western blot. * p < 0.05, ** p < 0.01