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Primers utilized for qRT-PCR
| Target | Primer sequence (5ʹ-3ʹ) |
|---|---|
| TBX1 | Forward: CAACAACCTACTGGACGACAACG |
| Reverse: CTCCTCGGCATATTTCTCGCTAT | |
| GAPDH | Forward: GACCTGACCTGCCGTCTAG |
| Reverse: AGGAGTGGGTGTCGCTGT | |
| U6 | Forward: GCTTCGGCAGCACATATACT |
| Reverse: GTGCAGGGTCCGAGGTATTC | |
| miR-6727-5p | Forward: ATCTCGGGGCAGGCGGCT |
| Reverse: GCAGGGTCCGAGGTATTC |
Note: qRT-PCR, quantitative real-time PCR; TBX1, T-box transcription factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-6727-5p, microRNA-6727-5p.
