Table 2.
Antibodies utilized for Western blot
| Antibody name | Dilution | Manufacturer | Cat |
|---|---|---|---|
| p-AKT (Ser473) | 1:1000 | CST | #4060 |
| AKT | 1:2000 | CST | #4691 |
| p-ERK1/2 (Thr202/Tyr204) | 1:1000 | Affinity | AF1015 |
| ERK1/2 | 1:1000 | Affinity | AF0155 |
| p-p38 (Thr180/Tyr182) | 1:500 | Affinity | AF4001 |
| p38 | 1:500 | Affinity | AF6456 |
| p-JNK (Thr183+ Tyr185) | 1:1000 | Affinity | AF3318 |
| JNK | 1:2000 | Affinity | AF6318 |
| TBX1 | 1:1000 | Affinity | AF0327 |
| GAPDH | 1:10,000 | Proteintech | 60,004-1-Ig |
| Goat anti rabbit IgG-HRP | 1:3000 | Solarbio | SE134 |
| Goat anti mouse IgG-HRP | 1:3000 | Solarbio | SE131 |
Note: AKT, protein kinase B; ERK, extracellular signal-regulated kinase; JNK, c-Jun NH2-terminal kinase; TBX1, T-box transcription factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IgG, immunoglobulin G; HRP, horseradish peroxidase.