Figure 2.

Analysis of H9 human embryonic stem cells (hESCs) growth dynamics and median fluorescence intensity (MedFI) values for OCT3/4, Ki67 and PAX6 markers for cells seeded at three different densities. (a) Specific growth rate (SGR) increased from negative SGR to positive SGR from day two to day four for all three densities. (b) Cell number decreased from day 0 to day two and then increased from day two four, the 10,000 cell/cm² density did not have a significant increase in cell number during the four-day culture period. (c) All three densities had a decrease in cell viability from day 0 to day two which increased from day two four. OCT3/4 (d) and Ki67(f) were both highly expressed. (e) PAX6 expression levels were lower in comparison to OCT3/4 and Ki67. Error bars indicate standard deviation, n = 3. ‘*’ indicates p < 0.05, ‘**’ indicates p < 0.01, ‘***’ indicates p < 0.001, and ‘****’ indicates p < 0.0001