Table 1.

Details of the different experimental culture routes investigated in experiment B: the effect of feeding regimes. The Control route was subjected to daily medium exchanges; Route 1 was subjected to a medium exchange on day one only; Route 2 underwent a medium exchange on day 2; Route 3 cells had no medium exchange. n = 3 for each condition was used. M. Ex = medium exchange

Route	Day 0	Day 1	Day 2	Day 3	Summary
Control	Seed	M.Ex	M.Ex	Passage/Harvest	Daily M.Ex
1	Seed	M.Ex		Passage/Harvest	M.ExEx on day 1 only
2	Seed		M.Ex	Passage/Harvest	M.Ex on day 2 only
3	Seed			Passage/Harvest	No M.Ex