Table 2.
Route | Day 0 | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 6 | Day 7 |
---|---|---|---|---|---|---|---|---|
Daily Feed | Seed | M.Ex | M.Ex | M.Ex | M.Ex | M.Ex | M.Ex | Endpoint harvest |
One Feed | Seed | M.Ex | Endpoint harvest |
N.B. the cells were cultured for seven days (more than double the standard culture length of three days) to push the cells to their growth and metabolic limits using the OF route.