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. 2021 Mar 24;12(1):979–996. doi: 10.1080/21655979.2021.1902696

Table 2.

Details of the two conditions used to investigate density and nutrient availability-based growth inhibition. Cells cultured following the Daily Feed (DF) route underwent medium exchange every 24 h; cells cultured under the one feed (OF) route underwent a single medium exchange following the first 24 h culture Sacrificial wells were harvested and counted daily: n= 3 for each condition was used. M. Ex = medium exchange; DF = daily feed; OF = one feed

Route Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
Daily Feed Seed M.Ex M.Ex M.Ex M.Ex M.Ex M.Ex Endpoint harvest
One Feed Seed M.Ex           Endpoint harvest

N.B. the cells were cultured for seven days (more than double the standard culture length of three days) to push the cells to their growth and metabolic limits using the OF route.