Figure 4.

Inhibiting circRNA_0082835 and/or EZH2 blocks Wnt/β-catenin, suppresses cell cycle-related protein expression and attenuates EMT in melanoma cells. (a) Relative protein expressions of Wnt/β-catenin signaling-related Wnt5a and β-catenin, cell cycle-related CyclinD1 and CyclinE1 in WM451 cells before and after transfection of shRNA-circ and/or shRNA-EZH2, detected by western blot. (b) Relative protein expressions of EMT-related CRKL, E-cad and N-cad in WM451 cells before and after transfection of shRNA-circ and/or shRNA-EZH2, detected by western blot. *P < 0.05, **P < 0.01 and ***P < 0.01 vs. Control group. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. shRNA-NC group. ^$P < 0.05, ^$$P < 0.01 and ^$$$P < 0.001 vs. shRNA-circ group. ^&&p < 0.01 and ^&&&p < 0.001 vs. shRNA-EZH2 group