Figure 5.

MiRNA-429 binds to circRNA_0082835 and its inhibition reverses the anti-proliferation effect of cicrRNA_0082835 knockdown. (a) Relative expression of miR-429 in WM451 cells before and after transfection of shRNA-circ and/or shRNA-EZH2, detected by qPCR. ***P < 0.001 vs. Control group. ^###P < 0.001 vs. shRNA-NC group. ^&&&P < 0.001 vs. shRNA-EZH2 group. (b-c) Relative luciferase activity in wt-circ + mimic NC, mut-circ + mimic NC, wt-circ + miR-429 mimic, wt-EZH2 + mimic NC, mut-EZH2 + mimic NC, wt-EZH2 + miR-429 mimic and mut-EZH2 + miR-429 mimic, detected by dual-luciferase reporter assay. ***P < 0.001 vs. wt-circ group. (d-e) Relative expression of miR-429 in WM451 cells before and after the transfection of miR-429 inhibitor in shRNA-circ groups and shRNA-EZH2 groups was detected by qPCR. **P < 0.01 and ***P < 0.001 vs. shRNA-circ group. ^##P < 0.01 and ^###P < 0.001 vs. shRNA-circ +miR-429 inhibitor group. (f) OD values of WM451 cells before and after the transfection of miR-429 inhibitor in shRNA-circ groups and shRNA-EZH2 groups were detected by CCK-8. ***P < 0.001 vs. shRNA-circ group