Figure 6.

MiR-429 inhibitor affects cell cycle regulation and reverses the anti-invasion and anti-migration effects of circRNA_0082835 knockdown. (a) WM451 cell cycle distribution before and after transfection of miR-429 inhibitor in shRNA-circ groups and shRNA-EZH2 groups, detected by flow cytometry. (b) Relative invasive WM451 cells before and after transfection of miR-429 inhibitor in shRNA-circ groups and shRNA-EZH2 groups, detected by transwell. (c) Relative wound width of WM451 cells before and after transfection of miR-429 inhibitor in shRNA-circ groups and shRNA-EZH2 groups, detected by wound healing. ***P < 0.001 vs. shRNA-circ group. ^##P < 0.01 and ^###P < 0.001 vs. shRNA-circ +miR-429 inhibitor group. ^$$P < 0.01 vs. shRNA-EZH2 group. ^&&&P < 0.001 vs. shRNA-EZH2 + miR-429 inhibitor group