Figure 2.

AK006774 inhibits the H/R-induced oxidative stress and apoptosis in vitro. (a) qPCR was used to evaluate the efficiency of AK006774-overexpressing adenovirus. (b-d) The level of LDH, MDA, and ROS were detected using commercial kits. (e) TUNEL staining was carried out to detect the apoptosis of cardiomyocytes. (f) Flow cytometry and Annexin V/PI cardiomyocytes staining were performed to detect the apoptosis of cardiomyocytes. *p < 0.05 vs control group, **p < 0.01 vs Ad-nc or control group, ^#p < 0.05 vs H/R+ Ad-nc group