Figure 6.

Downregulation of miR-486-5p inactivates the TGF-β/Smad pathway by regulation of PIM1. (a) The levels of TGF-β and phosphorylated (and total) Smad3 in L02 cells in 4 groups: H₂O₂; H₂O₂ + UCB-MSC-CM; H₂O₂ + UCB-MSC-CM + miR-486-5p inhibitor; H₂O₂ + UCB-MSC-CM + si-PIM1 were detected using western blot analysis. (b) The levels of TGF-β and phosphorylated (and total) Smad3 in L02 cells in 6 groups: Control; H₂O₂; H₂O₂ + NC; H₂O₂ + miR-486-5p inhibitor; H₂O₂ + si-PIM1; H₂O₂ + miR-486-5p inhibitor+si-PIM1 groups. *P < 0.05