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. 2021 Aug 10;12(1):4911–4923. doi: 10.1080/21655979.2021.1959865

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Induction of BMSCs osteogenic differentiation. (a) BMSCs’ cell morphology switched from elongated fusiform to polygonal (Magnification: 200×, scale bar: 250 μm). (b) Alizarin Red staining showed increased mineralized nodules formation (Magnification: 200×, scale bar: 250 μm). (c) ALP staining showed increased ALP protein expression (Magnification: 200×, scale bar: 250 μm). (d) ALP activity tests showed increased ALP activity (***p < 0.001). (e) qRT-PCR showed increased the mRNA expression of RUNX2, OPN, and OCN (**p < 0.01, ****p < 0.0001). Non-ind: Non-induced group; Ind: Induced group