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. 2021 Aug 10;12(1):4911–4923. doi: 10.1080/21655979.2021.1959865

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effects of circ_0005564 on the expression of osteogenic markers. (a) siRNAs was transfected into BMSCs, and circRNAs expression was tested by qRT-PCR. *p < 0.05. (b) RUNX2, OPN, and OCN were detected by qRT-PCR after circ_0005564 silencing. *p < 0.05. (c) The formation pattern of circ_0005564. (d) Divergent and convergent primers were designed to determine the circular form of circ_0005564. PCR products were observed through agarose gel electrophoresis. (e) Sanger sequencing of products from divergent primers of circ_0005564