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. 2021 May 28;12(1):2095–2105. doi: 10.1080/21655979.2021.1931644

Figure 3.

Figure 3.

Verification of virus packaging capability of 293 T suspension cells. (a) observation of GFP expression in 293 T suspension cells after infection using 100 µm for green field microscopy. (b) 100 µL cell supernatant was collected, treated at 72 h post-transduction, and tested using an HIV p24 Antigen Rapid Test Cassette. the test shows positive results (2 horizontal lines). (c, d) inoculated 293 T cells with 6 increasing amounts (0–64 µL) of the supernatant were detected using a flow cytometer