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Sequences of amplification primer
| Gene | Sequence (5'–3') | |
|---|---|---|
| miR-221 | Forward | ACCTGGCATACAATGTAG |
| Reverse | GAACATGTCTGCGTATCTC | |
| U6 | Forward | UUCUCCGAACGUGUCACGUTT |
| Reverse | UGACACGUUCGGAGAATT | |
| PIK3R1 | Forward | CGCCTCTTCTTATCAAGCTCGTG |
| Reverse | GAAGCTGTCGTAATTCTGCCAGG | |
| GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT |
| Reverse | GGCTGTTGTCATACTTCTCATGG |
