Figure 3.

EV-miR-23b improves motor dysfunction and tissue damage of hindlimbs in SCI rats. (a) RT-qPCR analysis of the expression of miR-23b in BMSCs and EVs, and the expression of miR-23b in the spinal cord tissues of each group of rats on day 7 after modeling; (b) Motor recovery of hindlimbs of SCI rats assessed by BBB scoring at before modeling and days 7, 14, 21, and 28 after modeling (N = 24/12); (c) Nissl staining analysis of the neuronal damage in the spinal cord tissues of each group of rats on days 7 and 28 after modeling and the number of Nissl stained-neurons in the spinal cord; (d) HE staining of the pathological changes in the spinal cord of each group of rats on days 7 and 28 after modeling; (e) ELISA analysis of the protein content of IL-6, IL-1β, TNF-α and IL-10 in the spinal cord of each group of rats on days 7 and 28 after modeling; (f) Western blot analysis of the expression of iNOS and Arg1 in the spinal cord of each group of rats on days 7 and 28 after modeling. N = 6. Data are expressed as mean ± standard deviation, and analyzed using one-way ANOVA and Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01