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. 2021 Oct 6;12(1):7563–7576. doi: 10.1080/21655979.2021.1980644

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — PFKFB3 silencing suppresses the proliferation of CSCC cells. (a) PFKFB3 mRNA expression and (b) PFKFB3 protein expression in A431 cells after transfection with shRNA-PFKFB3#1/2 was respectively tested by RT-qPCR and Western blot. (c) The viability, (d) proliferation and (e) Ki-67 expression of A431 cells transfected with shRNA-PFKFB3#2 was in turn analyzed by MTT assay, clone formation assay and immunofluorescence assay. *P < 0.05 and ***P < 0.001 vs. Control. ^##P < 0.01 and ^###P < 0.001 vs. shRNA-NC. ^@@P < 0.01 vs. shRNA-PFKFB3#1