Figure 6.

KLF9 knockdown attenuated the impacts of PFKFB3 knockdown on the proliferation of CSCC cells. (a) PFKFB3 mRNA expression and (b) PFKFB3 protein expression in A431 cells transfected with shRNA-PFKFB3#2 and shRNA-KLF9#2 was respectively detected using RT-qPCR and Western blot. (c) The viability, (d) proliferation and (e) Ki-67 expression of A431 cells transfected with shRNA-PFKFB3#2 and shRNA-KLF9#2 was tested with MTT assay, clone formation assay and immunofluorescence analysis. *P < 0.05 and ***P < 0.001 vs. Control. ^#P < 0.05 and ^###P < 0.001 vs. shRNA-NC. ^@P < 0.05 and ^@@@P < 0.001 vs. shRNA-PFKFB3. ^$P < 0.05 and ^$$$P < 0.001 vs. shRNA-PFKFB3 + shRNA-NC