Figure 7.

KLF9 knockdown reversed the inhibitory effect of PFKFB3 knockdown on the metastasis of CSCC cells. (a) The migration and (b) invasion of A431 cells transfected with shRNA-PFKFB3#2 and shRNA-KLF9#2 were assessed with wound healing assay and transwell assays. The expression of (c) migration and invasion related proteins and (d) EMT related proteins in A431 cells transfected with shRNA-PFKFB3#2 and shRNA-KLF9#2 was examined by Western blot. ***P < 0.001 vs. Control. ^###P < 0.001 vs. shRNA-NC. ^@P < 0.05, ^@@P < 0.01 and ^@@@P < 0.001 vs. shRNA-PFKFB3. ^$P < 0.05, ^$$P < 0.01 and ^$$$P < 0.001 vs. shRNA-PFKFB3 + shRNA-NC