Fig. 4.
Fluorescein diffusion analysis using the 3D-printed hydrogel partitioning chip with a 10% PEG-DA-700 hydrogel barrier. (A) Fluorescence image of fluorescein (colored in green) diffusion at 0 min and 90 min through the 10% PEG-DA-700 hydrogel barrier. The dotted line depicts the boundary of the hydrogel barrier channel. (B) A plot of the characteristic diffusion length of fluorescein across the 10% PEG-DA-700 hydrogel barrier as a function of the square root of time (dots), the green line is a linear fit of the dots, L = 5.31 t1/2 (adjusted R2 = 0.96).