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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Lab Chip. 2019 Sep 10;19(18):3086–3093. doi: 10.1039/c9lc00535h

Fig. 5.

Fig. 5.

Diffusion analysis of fluorescein (green), 10k-dextran-Alexa 680 (purple), and BSA-Texas Red (red) using the 3D-printed hydrogel partitioning chip with a 2% agarose hydrogel barrier. (A-B) Fluorescence image of fluorescein (colored in green), 10k-dextran-Alexa 680 (colored in magenta), and BSA-Texas Red (colored in red) diffusion at 0 min and 10 min through the 2% agarose hydrogel barrier. (C) A plot of the characteristic diffusion length of fluorescein, 10k-dextran-Alexa 680, and BSA-Texas Red across the 2% agarose hydrogel barrier as a function of the square root of time (dots). The green line is a linear fit of the green dots (diffusion length of fluorescein), L = 32.62729 t1/2 (adjusted R2 = 0.99584). The magenta line is a linear fit of the magenta dots (diffusion length of 10k-dextran-Alexa 680), L = 14.44622 t1/2 (adjusted R2 = 0.99815). The red line is a linear fit of the red dots (diffusion length of BSA-Texas Red), L = 12.68639 t1/2. (adjusted R2 = 0.98712).