Figure 3.

Effect of MCM3AP-AS1 and miR-195-5p on A549 and H226 cells proliferation and apoptosis

(a) MCM3AP-AS1 overexpressing plasmid and miR-195-5p mimics, sh-MCM3AP-AS1#1 and miR-195-5p inhibitors were co-transfected into A549 and H226 cells, respectively. qRT-PCR was used to detect transfection efficiency. (b) After the transfection, CCK-8 method was applied to detect the cell viability of A549 and H226 cells. (c–d) After the transfection, EdU assay was utilized to detect the proliferation of A549 and H226 cells. (e–f) After the transfection, Western blot was employed to detect the Bax and Bcl-2 expressions. All of the experiments were performed in triplicate. *P < 0.05, **P < 0.01 and *** P < 0.001.