Figure 5.

MCM3AP-AS1 up-regulated E2F3 expression by adsorbing miR-195-5p

(a–b) The schematic map of the E2F3 3ʹUTR WT and E2F3 3ʹUTR MUT binding site for miR-195-5p, which was predicted by TargetScan database (Context score percentile: 95). (c) E2F3 3ʹUTR-WT or E2F3 3ʹUTR-MUT was co-transfected into HEK-293 T cells with miR-195-5p mimics or miR-195-5p inhibitors, and the relative luciferase activity was measured. (d–e) MCM3AP-AS1 overexpressing plasmid and miR-195-5p mimics, sh-MCM3AP-AS1#1 and miR-195-5p inhibitors were co-transfected into A549 and H226 cells, respectively. qRT-PCR and Western blot were used to detect the expression of E2F3 in NSCLC cells. All of the experiments were performed in triplicate. ** P < 0.01, *** P < 0.001.