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. 2021 Oct 27;12(1):8217–8232. doi: 10.1080/21655979.2021.1982272

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — DARS-AS1 targeted miR-3200-5p, and the latter targeted CKAP2

A-B. The potential miRNAs binding to DARS-AS1 and CKAP2 were predicted by the Starbase database. As a result, miR-3200-5p had a common binding site with DARS-AS1 and CKAP2. C-D. The wild-type or mutated dual-luciferase reporter gene vectors of DRAS-1 and CKAP2 and miR-3200-5p mimics were transfected into Huh7 cells. E. The miR-3200-5p expression in DARS-AS1-overexpressed HCC cells was examined by RT-qPCR. F. HCC cells overexpressing miR-3200-5p were constructed by transfecting the HCC cells with miR-3200-5p mimics. G. RT-qPCR was applied to test the mRNA level of CKAP2 in the miR-3200-5p overexpression model. NS, *** indicated P> 0.05,P< 0.001. N = 3.