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. 2021 Oct 27;12(1):8217–8232. doi: 10.1080/21655979.2021.1982272

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — DARS-AS1 weakened the tumor-suppressive effect mediated by miR-3200-5p overexpression

miR-3200-5p mimics or DARS-AS1 overexpression plasmids were transfected into Huh7 cells. A-D. RT-qPCR and WB were utilized to check DARS-AS1 (A), miR-3200-5p (B), CKAP2 (C) and FAK/ERK (D) levels. E. CCK-8 was performed to verify Huh-7 and HCC-LM3 cell proliferation. F. Cell proliferation was examined by the colony formation assay. G. Transwell assay was implemented to test cell invasiveness. H. The expression of E-cadherin, N-cadherin and Vimentin in HCC cells was compared by WB. NS, *, **, *** indicated P> 0.05,P< 0.05,P< 0.01,P< 0.001. N= 3.