Figure 3.

FOXD3-AS1 up-regulates TFF1 expression through sponging miR-363. A, the sub-cellular location of FOXD3-AS1 was determined by FISH assay. B-C, the binding relationship between FOXD3-AS1 and miR-363 was predicted on TargetScan (http://www.targetscan.org/vert_72/) (b) and validated by a dual luciferase reporter gene assay (c). D-E, the binding relationship between miR-363 and TFFI mRNA was predicted on TargetScan (d) and validated by a dual luciferase reporter gene assay (e). F-G, expression of miR-393 (f) and TFF1 mRNA (g) in ER+ and ER- cells was determined by RT-qPCR. H, miR-363 expression in MDA-MB-231 and T47D cells after FOXD3-AS1 interference was determined by RT-qPCR. I, protein level of TFF1 in MDA-MB-231 and T47D cells after FOXD3-AS1 interference was detected by western blot analysis. Repetition = 3. Data were exhibited as mean ± SD. Data in panels B and E were analyzed using two-way ANOVA while data in panels F, G, H and I were analyzed using one-way ANOVA, and Tukey’s multiple comparison test was used for post hoc test. *, p < 0.05