Figure 2.

RECQL4 deletion inhibited the proliferation, migration and invasion of HCC cells. (a-b) RT-qPCR and western blot analysis were utilized for detection of RECQL4 expression after transfection with si-RECQL4#1 or si-RECQL4#2 in HuH-7 cells. ***P < 0.001. (c) cell viability was assessed with CCK-8 assay. *P < 0.05, ***P < 0.001 vs. si-NC. (d) the capacity of cell migration was tested by wound healing assay. (e) transwell assay was adopted for determining cell invasion. (f) the expression of E-cadherin, N-cadherin and vimentin was tested with western blotting. ***P < 0.001