Figure 4.

SRSF1 overexpression crippled the repressive impact of RECQL4 silencing on the proliferation, migration and invasion of HCC cells. (a-b) RECQL4 levels were assessed using RT-qPCR and western blot assay after transfection with RECQL4 plasmid. (c-d) RT-qPCR and western blotting were conducted to assess RECQL4 expression after transfection with si-RECQL4 and Oe-SRSF1. **P < 0.01, ***P < 0.001. (e) cell viability was tested with CCK-8 kit. *P < 0.05, ***P < 0.001 vs. si-NC; ^#P < 0.05, ^###P < 0.001 vs. si-RECQL4+ oe-NC. (f) the capacity of cell migration was tested by wound healing assay. (g) transwell assay was adopted for measuring cell invasion. (h) the expression of E-cadherin, N-cadherin and vimentin was tested with western blotting. *P < 0.05. **P < 0.01, ***P < 0.001