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Primer sequences for RT-qPCR
| Gene | Gene Primer sequence (5ʹ-3ʹ) |
|---|---|
| miR-495 | Forward: 5ʹ- ACACTCCAGCTGGGGAAGTTGCCCATGTT −3ʹ Reverse: 5ʹ- CTCAACTGGTGTCGTGGA −3’ |
| CTRP3 | Forward: 5ʹ- TACCTTATGCACAATGGCAACA −3ʹ Forward: 5ʹ- AGCATGATTGCTGGATGTATCTG −3’ |
| GAPDH | Forward: 5ʹ-GAGTCAACGGATTTGGTCGT-3ʹ Reverse: 5ʹ-TTGATTTTGGAGGGATCTCG-3’ |
| U6 | Forward: 5ʹ-TTGGTCTGATCTGGCACATATAC-3ʹ Reverse: 5ʹ-AAAAATATGGAGCGCTTCACG-3’ |
