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. 2021 Oct 27;12(1):8753–8767. doi: 10.1080/21655979.2021.1982230

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — KCNQ1OT1 decoyed miR-296-5p to up-regulate its target gene HYOU1 in CC. (a) starBase predicted the complementary binding sites of miR-296-5p and HYOU1. (b) Dual-luciferase reporter assay confirmed the binding relationship between miR-296-5p and HYOU1. (c) RIP assay analyzed the potential binding between miR-296-5p and HYOU1. (d) Western blotting analysis examined the protein level of HYOU1 in CC cells after transfection with miR-296-5p mimic. (e) RT-qPCR detected the mRNA level of HYOU1 in CC cells after transfection with miR-296-5p mimic. (f) Western blotting analysis examined the protein level of HYOU1 in CC cells after transfection with pcDNA-KCNQ1OT1 or co-transfection with miR-296-5p mimic. (g) RT-qPCR detected the mRNA level of HYOU1 in CC cells after transfection with pcDNA-KCNQ1OT1 or co-transfection with miR-296-5p mimic