Figure 5.

miR-25-3p enhanced chemoresistance of NSCLC cells. (a) miR-25-3p mimics ameliorated IC50 value. (b) Colony forming assay was used to detect miR-25-3p restrainer role on cell multiplication. (c) DDP induced apoptotic of A549 cells infected with miR-25-3p over-expression via flow cytometry. (d) Cisplatin induced apoptotic of A549/DDP cells infected with miR-25-3p inhibitor via flow cytometry. (e) After miR-25-3p mimic/inhibitor/PTEN cotransfected, our crew detected PTEN, PI3K, p-AKT and AKT levels. (-), not treated with cisplatin (+), treated with cisplatin