Figure 5.

circBCL11B regulates LASP1 expression via miR-579

A Starbase predicted that miR-579 may bind to the 3 ‘noncoding region of LASP1. B Dual-luciferase reporter assay showed the luciferase activity of LASP1 3ʹUTR-WT and LASP1 3ʹUTR-MUT vector after transfecting with miR-579 mimic. C RNA pull-down was performed to detect the binding between miR-579 and circBCL11B or LASP1 (p < 0.01). D Western blotting showed the protein level of LASP1 in miR-579 overexpressed cells. E The effect of miR-579 inhibitor was detected by qRT-PCR (p < 0.01). F Protein levels of LASP1 in OSCO cells (si-NC, si-circBCL11B, si-circBCL11B+miR-579 inhibitor) were detected by Western blotting. Data are summary of three independent experiment.