Figure 6.

MiR-1224-5p overexpression overcomes 5-FU resistance in 5-FU-resistant OSCC cells

(A) CCK-8 revealed the IC₅₀ value in HSC-3/5-FU and HN-4/5-FU cells transfected with NC mimics or miR-1224-5p mimics. (B) Colony formation assay were used to test cell proliferation and HSC-3/5-FU and HN-4/5-FU cells treated with NC mimics or miR-1224-5p mimics. (C) Flow cytometry analysis was applied to examine apoptosis in HSC-3/5-FU and HN-4/5-FU cells transfected with NC mimics or miR-1224-5p mimics. (D-F) CCK-8, Colony formation assay and flow cytometry were employed to detect IC₅₀, cell proliferation and apoptosis in HSC-3/5-FU and HN-4/5-FU cells transfected with NC inhibitor or miR-1224-5p inhibitor. *P< 0.05, **P < 0.01.