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. 2021 Jun 2;12(1):2299–2310. doi: 10.1080/21655979.2021.1934241

Figure 4.

Figure 4.

ZFPM2-AS1 interacted with miR-515-5p. (a) Potential miRNAs that had complementary base for ZFPM2-AS1 were obtained from starBase, and the interaction between ZFPM2-AS1 and these miRNAs was validated by RNA pull-down assay. (b) MiR-515-5p expression in glioma tissues and cells. (c) The transfection efficiency of miR-515-5p was confirmed by RT-qPCR. (d) Luciferase reporter assay was performed to verify the binding site between miR-515-5p and ZFPM2-AS1. (e) RIP assay was conducted to testify the interaction between miR-515-5p and ZFPM2-AS1. (f) Expression association between miR-515-5p and ZFPM2-AS1 was validated by Pearson’s association analysis. *p < 0.05, **p < 0.01, ***p < 0.001