Figure 1.

LUM expression was notably decreased in TNF-α-induced hNPCs and LUM deletion reduced TNF-α-induced inflammatory response in hNPCs. (a) Detection of LUM protein expression by means of western blot in the control group and in hNPCs stimulated with TNF-α. N = 3. ***P < 0.001 vs. control. (b–c) Detection of the interference efficacy of si-LUM1/2 by means of RT-qPCR and western blot analysis in hNPCs. N = 3. **P < 0.01, ***P < 0.001 vs. si-NC. (d–f) The concentrations of pro-inflammatory cytokine levels in hNPCs stimulated with TNF-α or transfected with si-NC/si-LUM-1was examined with ELISA kits. (g–i) RT-qPCR was used to evaluate the mRNA expression of pro-inflammatory cytokine in hNPCs stimulated with TNF-α or transfected with si-NC/si-LUM-1. N = 3. ***P < 0.001 vs. control; ^###P < 0.001 vs. TNF-α+ si-NC